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Comparison between Newly Developed and Commercial Inhalant Skin Prick Test Reagents Using In Vivo and In Vitro Methods
Article in En | WPRIM | ID: wpr-713707
Responsible library: WPRO
ABSTRACT
BACKGROUND: We developed skin prick test (SPT) reagents for common inhalant allergens that reflected the real exposure in Korea. The study aim was to evaluate diagnostic usefulness and allergen potency of our inhalant SPT reagents in comparison with commercial products. METHODS: We produced eight common inhalant allergen SPT reagents using total extract (Prolagen): Dermatophagoides farinae, Dermatophagoides pteronyssinus, oak, ragweed, mugwort, Humulus japonicus pollens, as well as cat and dog allergens. We compared the newly developed reagents with three commercially available SPT reagents (Allergopharma, Hollister-Stier, Lofarma). We measured total protein concentrations, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), major allergen concentration, and biological allergen potencies measured by immunoglobulin E (IgE) immunoblotting and ImmunoCAP inhibition test. RESULTS: Diagnostic values of these SPT reagents were expressed as positivity rate and concordance rate of the results from ImmunoCAP allergen-specific IgE test in 94 allergic patients. In vitro analysis showed marked differences in protein concentrations, SDS-PAGE features, major allergen concentrations, and biological allergen potencies of four different SPT reagents. In vivo analysis showed that positive rates and concordance rates of Prolagen® SPT reagents were similar compared to the three commercial SPT reagents. CONCLUSION: The newly developed Prolagen® inhalant SPT reagents are not inferior to the commercially available SPT reagents in allergy diagnosis.
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Full text: 1 Index: WPRIM Main subject: Pollen / Skin / Sodium / In Vitro Techniques / Immunoglobulin E / Immunoglobulins / Allergens / Immunoblotting / Artemisia / Humulus Type of study: Diagnostic_studies Limits: Animals / Humans Country/Region as subject: Asia Language: En Journal: Journal of Korean Medical Science Year: 2018 Type: Article
Full text: 1 Index: WPRIM Main subject: Pollen / Skin / Sodium / In Vitro Techniques / Immunoglobulin E / Immunoglobulins / Allergens / Immunoblotting / Artemisia / Humulus Type of study: Diagnostic_studies Limits: Animals / Humans Country/Region as subject: Asia Language: En Journal: Journal of Korean Medical Science Year: 2018 Type: Article