Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells
Immune Network
; : e16-2018.
Article
in En
| WPRIM
| ID: wpr-714172
Responsible library:
WPRO
ABSTRACT
Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naïve T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner. We found that, except for programmed death-ligand 1, most cell surface molecules were expressed lower in the BM cells cultured with GM-CSF for the extended duration. These results indicate that BM cells in the extended culture with GM-CSF undergo 2 distinct steps of functional change; first, they lose the immunostimulatory capacity; and next, they gain the immunosuppressive ability.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Bone Marrow
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Dendritic Cells
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T-Lymphocytes
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Granulocyte-Macrophage Colony-Stimulating Factor
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Immunosuppression Therapy
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Lymphocyte Culture Test, Mixed
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Granulocytes
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Major Histocompatibility Complex
Language:
En
Journal:
Immune Network
Year:
2018
Type:
Article