Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani
The Korean Journal of Parasitology
;
: 221-231, 2003.
Article
in English
| WPRIM
| ID: wpr-7143
ABSTRACT
To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paragonimus
/
Phylogeny
/
Molecular Sequence Data
/
Sequence Alignment
/
Amino Acid Sequence
/
RNA-Directed DNA Polymerase
/
Genome
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Retroelements
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS