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ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas
The Korean Journal of Parasitology ; : 203-207, 2003.
Article in English | WPRIM | ID: wpr-7145
ABSTRACT
An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used as template to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1). Each amplified DNA fragment was inserted into pQE30 plasmid to induce the expression of His-tagged protein in Escherichia coli (M15 strain) by IPTG. His-tagged proteins were purified by Ni-NTA metal-affinity chromatography and used as antigens for ELISA with patient sera that were confirmed previously by blood smear examinations. When applied to patient sera, 122 (80.3%) out of 152 vivax malaria cases reacted to at least one antigen, while no reactions were observed with 128 uninfected serum samples. We applied this ELISA to the screening of 3, 262 civilian residents in endemic regions near the DMZ, which resulted in 236 positively detected (7.2%) cases. This method can be applied to serological diagnosis and mass screening in endemic regions, or can be used as a safety test for transfusion blood in endemic areas.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmodium vivax / Recombinant Proteins / Enzyme-Linked Immunosorbent Assay / Serologic Tests / Antibodies, Protozoan / Mass Screening / Malaria, Vivax / Endemic Diseases / Life Cycle Stages / Antigens, Protozoan Type of study: Diagnostic study / Prognostic study / Screening study Limits: Animals / Humans Language: English Journal: The Korean Journal of Parasitology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmodium vivax / Recombinant Proteins / Enzyme-Linked Immunosorbent Assay / Serologic Tests / Antibodies, Protozoan / Mass Screening / Malaria, Vivax / Endemic Diseases / Life Cycle Stages / Antigens, Protozoan Type of study: Diagnostic study / Prognostic study / Screening study Limits: Animals / Humans Language: English Journal: The Korean Journal of Parasitology Year: 2003 Type: Article