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Biological and Genetic Characteristics of Clinically Isolated Enterobacter cloacae with Multidrug Resistance
Laboratory Medicine Online ; : 99-106, 2018.
Article in English | WPRIM | ID: wpr-715910
ABSTRACT

BACKGROUND:

From January 2014 to December 2015, 69 clones of Enterobacter cloacae showing multidrug resistance to six classes of antimicrobial agents were collected from two medical centers in Korea.

METHODS:

Minimum inhibitory concentrations were determined using the E-test method, and 17 genes were detected using polymerase chain reaction (PCR). The epidemiological relatedness of the strains was identified using repetitive element sequence-based PCR and multilocus sequence typing.

RESULTS:

The 69 E. cloacae clones produced extended spectrum β lactamase (ESBL) and AmpC and showed multidrug resistance to cefotaxime, ceftazidime, and aztreonam. We identified the following sequence types ST56 of type VI for ESBL SHV (N=12, 17.4%); ST53, ST114, ST113, and ST550 of types I, IV, VI, and VII, respectively, for CTX-M (N=11, 15.9%); and ST668 of type III for the carbapenemase NDM gene (N=1, 1.5%). The AmpC DHA gene (N=2, 2.89%) was confirmed as ST134, although its type was not identified, whereas EBC (MIR/ACT; N=18, 26.1%) was identified as ST53, ST24, ST41, ST114, ST442, ST446, ST484, and ST550 of types V, I, III, IV, VII, and VI, respectively. The formed subclasses were bla CTX-M-3 and bla CTX-M-22 by CTX-M-1, bla CTX-M-9 and bla CTX-M-125 by CTX-M-9, bla DHA-1 by DHA, and bla MIR-7 and bla ACT-15,17,18,25,27,28 by EBC (MIR/ACT).

CONCLUSIONS:

There were no epidemiological relationships between the gene products and the occurrence of resistance among the strains.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Aztreonam / Microbial Sensitivity Tests / Cefotaxime / Ceftazidime / Polymerase Chain Reaction / Cloaca / Enterobacter cloacae / Clone Cells / Drug Resistance, Multiple / Enterobacter Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Laboratory Medicine Online Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Aztreonam / Microbial Sensitivity Tests / Cefotaxime / Ceftazidime / Polymerase Chain Reaction / Cloaca / Enterobacter cloacae / Clone Cells / Drug Resistance, Multiple / Enterobacter Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Laboratory Medicine Online Year: 2018 Type: Article