Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts
Restorative Dentistry & Endodontics
;
: e24-2018.
Article
in English
| WPRIM
| ID: wpr-716156
ABSTRACT
OBJECTIVES:
The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. MATERIALS ANDMETHODS:
Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103/well, and were incubated for 24 hours with 11, 12, 14, and 18 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry.RESULTS:
The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 11 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry.CONCLUSIONS:
This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apicoectomy
/
Periodontal Ligament
/
Polytetrafluoroethylene
/
In Vitro Techniques
/
Cell Survival
/
Survival Rate
/
Cell Death
/
Apoptosis
/
Cell Culture Techniques
/
Polymethyl Methacrylate
Limits:
Humans
Language:
English
Journal:
Restorative Dentistry & Endodontics
Year:
2018
Type:
Article
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