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Interleukin-4 Contributes to Degeneration of Dopamine Neurons in the Lipopolysaccharide-treated Substantia Nigra in vivo
Experimental Neurobiology ; : 309-319, 2018.
Article in English | WPRIM | ID: wpr-716236
ABSTRACT
The present study investigated the effects of interleukin (IL)-4 on dopamine (DA) neurons in the substantia nigra (SN) in vivo of lipopolysaccharide (LPS)-treated rat. Tyrosine hydroxylase immunohistochemistry showed a significant loss of nigral DA neurons at 3 and 7 day post-LPS. In parallel, IL-4 immunoreactivity was upregulated as early as 1 day, reached a peak at 3 day and remained elevated at 7 day post-LPS. IL-4 immunoreactivity was detected exclusively in microglia. IL-4 neutralizing antibody (NA) significantly increased survival of DA neurons in LPS-treated SN in vivo by inhibiting microglial activation and production of proinflammatory mediator such as IL-1β as assessed by immunihistochemical, RT-PCR and ELISA analysis, respectively. Accompanying neuroprotection are IL-4NA effects on decreased disruption of blood-brain barrier and astrocytes. The present data suggest that endogenously expressed IL-4 from reactive microglia may be involved in the neuropathological processes of degeneration of DA neurons occurring in Parkinson's disease.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Parkinson Disease / Tyrosine 3-Monooxygenase / Enzyme-Linked Immunosorbent Assay / Substantia Nigra / Immunohistochemistry / Blood-Brain Barrier / Dopamine / Lipopolysaccharides / Astrocytes / Interleukins Limits: Animals Language: English Journal: Experimental Neurobiology Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Parkinson Disease / Tyrosine 3-Monooxygenase / Enzyme-Linked Immunosorbent Assay / Substantia Nigra / Immunohistochemistry / Blood-Brain Barrier / Dopamine / Lipopolysaccharides / Astrocytes / Interleukins Limits: Animals Language: English Journal: Experimental Neurobiology Year: 2018 Type: Article