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Viperin Deficiency Promotes Polarization of Macrophages and Secretion of M1 and M2 Cytokines
Immune Network ; : e32-2018.
Article in English | WPRIM | ID: wpr-716249
ABSTRACT
Viperin is a multifunctional protein that was first identified in human primary macrophages treated with interferon-γ and in human fibroblasts infected with human cytomegalovirus. This protein plays a role as an anti-viral protein and a regulator of cell signaling pathways or cellular metabolism when induced in a variety of cells such as fibroblasts, hepatocytes and immune cells including T cells and dendritic cells. However, the role of viperin in macrophages is unknown. Here, we show that viperin is basally expressed in murine bone marrow cells including monocytes. Its expression is maintained in bone marrow monocyte-derived macrophages (BMDMs) depending on macrophage colony-stimulating factor (M-CSF) treatment but not on granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment. In wild type (WT) and viperin knockout (KO) BMDMs differentiated with M-CSF or G-MCSF, there are little differences at the gene expression levels of M1 and M2 macrophage markers such as inducible nitric oxide synthase (iNOS) and arginase-1, and cytokines such as IL-6 and IL-10, indicating that viperin expression in BMDMs does not affect the basal gene expression of macrophage markers and cytokines. However, when BMDMs are completely polarized, the levels of expression of macrophage markers and secretion of cytokines in viperin KO M1 and M2 macrophages are significantly higher than those in WT M1 and M2 macrophages. The data suggest that viperin plays a role as a regulator in polarization of macrophages and secretion of M1 and M2 cytokines.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bone Marrow / Dendritic Cells / Bone Marrow Cells / Monocytes / T-Lymphocytes / Gene Expression / Cytokines / Granulocyte-Macrophage Colony-Stimulating Factor / Macrophage Colony-Stimulating Factor / Interleukin-6 Type of study: Prognostic study Limits: Humans Language: English Journal: Immune Network Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bone Marrow / Dendritic Cells / Bone Marrow Cells / Monocytes / T-Lymphocytes / Gene Expression / Cytokines / Granulocyte-Macrophage Colony-Stimulating Factor / Macrophage Colony-Stimulating Factor / Interleukin-6 Type of study: Prognostic study Limits: Humans Language: English Journal: Immune Network Year: 2018 Type: Article