IgE Cross-Reactivity between Humulus japonicus and Humulus lupulus
Yonsei Medical Journal
;
: 852-856, 2018.
Article
in English
| WPRIM
| ID: wpr-716927
ABSTRACT
PURPOSE:
Japanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The pollen of common hop (Humulus lupulus), a closely related species, may provide an alternative source if there is strong IgE cross-reactivity between these two species. We aimed to compare the IgE cross-reactivity and allergenicity of common hop and Japanese hop pollen. MATERIALS ANDMETHODS:
Cross-reactivity was measured by inhibition ELISA. One- and two-dimensional (2D) gel analyses combined with IgE immunoblotting and mass spectrometry [liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)] were performed to detect IgE-reactive pollen components.RESULTS:
Up to 16.7% of IgE reactivity to Japanese hop was inhibited by common hop. A 12-kDa protein component of Japanese hop pollen that showed the most potent IgE reaction was absent from common hop. Six IgE-reactive components from Japanese hop were detected by 2D gel electrophoresis and LC-ESI-MS/MS, but showed low Mascot scores, preventing positive identification.CONCLUSION:
No significant IgE cross-reaction was observed for Japanese and common hop pollen allergens. Development of allergy diagnostic and immunotherapeutic reagents based on Japanese hop pollen are urgently needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants
/
Pollen
/
Mass Spectrometry
/
Immunoglobulin E
/
Enzyme-Linked Immunosorbent Assay
/
Allergens
/
Electrophoresis, Gel, Two-Dimensional
/
Immunoblotting
/
Rhinitis, Allergic, Seasonal
/
Chromatography
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Yonsei Medical Journal
Year:
2018
Type:
Article
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