BubR1 Insufficiency Impairs Affective Behavior and Memory Function in Mice / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: S122-S130, 2018.
Article
in English
| WPRIM
| ID: wpr-717679
ABSTRACT
PURPOSE:
Although aging causes functional declines in cognition, the molecular mechanism underlying these declines remains largely unknown. Recently, the spindle checkpoint kinase budding uninhibited by benzimidazole-related 1 (BubR1) has emerged as a key determinant for age-related pathology in various tissues including brain. However, the neurobehavioral impact of BubR1 has not been explored. In this study, we investigated the role of BubR1 in behavioral function.METHODS:
To investigate the neurobiological functions of BubR1 in vivo, we utilized transgenic mice harboring BubR1 hypomorphic alleles (BubR1 H/H mice), which produce low amounts of BubR1 protein, as well as mice that have specific knockdown of BubR1 in the adult dentate gyrus. To assess anxiety-like behavior, the above groups were subjected to the elevated plus maze and the light-dark test, in addition to utilizing the tail-suspension and forced-swim test to determine depression-like behavior. We used novel object recognition to test for memory-related function.RESULTS:
We found that BubR1 H/H mice display several behavioral deficits when compared to wild-type littermates, including increased anxiety in the elevated-plus maze test, depression-like behavior in the tail suspension test, as well as impaired memory function in the novel object recognition test. Similar to BubR1 H/H mice, knockdown of BubR1 within the adult dentate gyrus led to increased anxiety-like behavior as well as depression-like behavior, and impaired memory function.CONCLUSIONS:
Our study demonstrates a requirement of BubR1 in maintaining proper affective and memory-related behavioral function. These results suggest that a decline in BubR1 levels with advanced age may be a crucial contributor to age-related hippocampal dysfunction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Anxiety
/
Pathology
/
Phosphotransferases
/
Brain
/
Aging
/
Mice, Transgenic
/
Cognition
/
Dentate Gyrus
/
Hindlimb Suspension
/
Alleles
Limits:
Animals
/
Humans
Language:
English
Journal:
International Neurourology Journal
Year:
2018
Type:
Article
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