The Suppressive Effect of Butyrate and Bromopyruvate on Inflammatory Cytokine Production and Short Chain Fatty Acid Receptor Expression by Blood Mononuclear Cells in Patients with Behçet's Disease
Annals of Dermatology
;
: 566-574, 2018.
Article
in English
| WPRIM
| ID: wpr-717766
ABSTRACT
BACKGROUND:
Controlling inflammation is a therapeutic goal of various autoimmune/autoinflammatory diseases including Behçet's disease (BD). The immunomodulatory effect of metabolites or metabolic analogs such as butyrate and 3-bromopyruvate has been observed in animal disease models.OBJECTIVE:
We attempted to evaluate the effect of butyrate and 3-bromopyruvate on the inflammatory cytokine production by peripheral blood mononuclear cells (PBMCs) isolated from patients with mucocutaneous involvement of BD.METHODS:
PBMCs isolated from 11 patients with BD and 10 healthy controls were stimulated with lipopolysaccharide in the presence of butyrate or 3-bromopyruvate. Butyrate receptor and cytokine messenger ribonucleic acid (mRNA) expression was analyzed by real-time reverse transcription polymerase chain reaction. Cytokine secretion was assessed by enzyme-linked immunosorbent assay. PBMCs survival was analyzed by flow cytometry.RESULTS:
Bromopyruvate or butyrate treatment suppressed inflammatory cytokine production in PBMCs from all our subjects. Bromopyruvate also reduced PBMCs survival while butyrate did not. As the effect of butyrate was slightly greater in BD patients than in healthy controls, we analyzed butyrate receptor expression and found that lipopolysaccharide-induced free fatty acid receptor 2 mRNA level in PBMCs was higher in BD patients than in controls.CONCLUSION:
We propose bromopyruvate and butyrate as supplementary therapeutic candidates to control inflammation in patients with BD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoimmune Diseases
/
Butyrates
/
RNA
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Polymerase Chain Reaction
/
Reverse Transcription
/
Disease Models, Animal
/
Flow Cytometry
/
Glycolysis
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2018
Type:
Article
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