Your browser doesn't support javascript.
loading
Effects of Several Cosmetic Preservatives on ROS-Dependent Apoptosis of Rat Neural Progenitor Cells
Biomolecules & Therapeutics ; : 608-615, 2018.
Article in English | WPRIM | ID: wpr-717991
ABSTRACT
Benzalkonium chloride, diazolidinyl urea, and imidazolidinyl urea are commonly used preservatives in cosmetics. Recent reports suggested that these compounds may have cellular and systemic toxicity in high concentration. In addition, diazolidinyl urea and imidazolidinyl urea are known formaldehyde (FA) releasers, raising concerns for these cosmetic preservatives. In this study, we investigated the effects of benzalkonium chloride, diazolidinyl urea, and imidazolidinyl urea on ROS-dependent apoptosis of rat neural progenitor cells (NPCs) in vitro. Cells were isolated and cultured from embryonic day 14 rat cortices. Cultured cells were treated with 1–1,000 nM benzalkonium chloride, and 1–50 μM diazolidinyl urea or imidazolidinyl urea at various time points to measure the reactive oxygen species (ROS). PI staining, MTT assay, and live-cell imaging were used for cell viability measurements. Western blot was carried out for cleaved caspase-3 and cleaved caspase-8 as apoptotic protein markers. In rat NPCs, ROS production and cleaved caspase-8 expression were increased while the cell viability was decreased in high concentrations of these substances. These results suggest that several cosmetic preservatives at high concentrations can induce neural toxicity in rat brains through ROS induction and apoptosis.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Stem Cells / Urea / Benzalkonium Compounds / In Vitro Techniques / Brain / Cell Survival / Cells, Cultured / Blotting, Western / Reactive Oxygen Species / Apoptosis Limits: Animals Language: English Journal: Biomolecules & Therapeutics Year: 2018 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Stem Cells / Urea / Benzalkonium Compounds / In Vitro Techniques / Brain / Cell Survival / Cells, Cultured / Blotting, Western / Reactive Oxygen Species / Apoptosis Limits: Animals Language: English Journal: Biomolecules & Therapeutics Year: 2018 Type: Article