Development of HLA-A, -B and -DR Typing Method Using Next-Generation Sequencing / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 310-319, 2018.
Article
in Korean
| WPRIM
| ID: wpr-718424
ABSTRACT
BACKGROUND:
Research on next-generation sequencing (NGS)-based HLA typing is active. To resolve the phase ambiguity and long turn-around-time of conventional high resolution HLA typing, this study developed a NGS-based high resolution HLA typing method that can handle large-scale samples within an efficient testing time.METHODS:
For HLA NGS, the condition of nucleic acid extraction, library construction, PCR mechanism, and HLA typing with bioinformatics were developed. To confirm the accuracy of the NGS-based HLA typing method, the results of 192 samples HLA typed by SSOP and 28 samples typed by SBT compared to NGS-based HLA-A, -B and -DR typing.RESULTS:
DNA library construction through two-step PCR, NGS sequencing with MiSeq (Illumina Inc., San Diego, USA), and the data analysis platform were established. NGS-based HLA typing results were compatible with known HLA types from 220 blood samples.CONCLUSION:
The NSG-based HLA typing method could handle large volume samples with high-throughput. Therefore, it would be useful for HLA typing of bone marrow donation volunteers.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Volunteers
/
Bone Marrow
/
Histocompatibility Testing
/
HLA-A Antigens
/
Gene Library
/
Polymerase Chain Reaction
/
Statistics as Topic
/
Computational Biology
/
Methods
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2018
Type:
Article
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