Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings
Blood Research
;
: 320-324, 2018.
Article
in English
| WPRIM
| ID: wpr-718477
ABSTRACT
BACKGROUND:
Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.METHODS:
MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH).RESULTS:
MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients.CONCLUSION:
Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prognosis
/
Leukemia, Lymphocytic, Chronic, B-Cell
/
Gene Expression
/
Polymerase Chain Reaction
/
Chromosome Aberrations
/
In Situ Hybridization
/
Cytogenetics
/
Reverse Transcription
/
RNA, Long Noncoding
/
Fluorescence
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Blood Research
Year:
2018
Type:
Article
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