Neutrophil oxidative burst as a diagnostic indicator of IgG-mediated anaphylaxis
Blood Research
;
: 299-306, 2018.
Article
in English
| WPRIM
| ID: wpr-718480
ABSTRACT
BACKGROUND:
IgG-mediated anaphylaxis occurs after infusion of certain monoclonal antibody-based therapeutics. New in vitro tests are urgently needed to diagnose such reactions. We investigated whether allergens trigger neutrophil oxidative burst (OB) and if neutrophil OB occurs due to allergen-specific IgG (sIgG).METHODS:
Neutrophil OB was measured by dihydrorhodamine 123 flow cytometry using a leukocyte suspension spiked with a very small patch of the allergen crude extract, Dermatophagoides farinae (Der f). The mean fluorescence intensity ratio of stimulated to unstimulated samples was calculated as the neutrophil oxidative index (NOI).RESULTS:
The Der f-specific NOI (Der f-sNOI) showed a time-dependent increase after Der f extract addition. At 15 min activation, higher Der f-sIgG levels were associated with lower Der f-sNOI values in 31 subjects (P < 0.05). This inverse relationship occurs due to the initial blocking effect of free Der f-sIgG. Additionally, neutrophil OB was nearly absent (Der f-sNOI of −1) in two cases a subject with undetectable Der f-sIgG levels and washed leukocyte suspensions deprived of Der f-sIgG.CONCLUSION:
Allergens can trigger neutrophil OB via preexisting allergen-sIgG. Neutrophil OB can be easily measured in a leukocyte suspension spiked with the allergen. This assay can be used to diagnose IgG-mediated anaphylaxis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Suspensions
/
In Vitro Techniques
/
Immunoglobulin G
/
Allergens
/
Respiratory Burst
/
Dermatophagoides farinae
/
Flow Cytometry
/
Fluorescence
/
Anaphylaxis
/
Leukocytes
Type of study:
Diagnostic study
Language:
English
Journal:
Blood Research
Year:
2018
Type:
Article
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