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Neutrophil oxidative burst as a diagnostic indicator of IgG-mediated anaphylaxis
Blood Research ; : 299-306, 2018.
Article in English | WPRIM | ID: wpr-718480
ABSTRACT

BACKGROUND:

IgG-mediated anaphylaxis occurs after infusion of certain monoclonal antibody-based therapeutics. New in vitro tests are urgently needed to diagnose such reactions. We investigated whether allergens trigger neutrophil oxidative burst (OB) and if neutrophil OB occurs due to allergen-specific IgG (sIgG).

METHODS:

Neutrophil OB was measured by dihydrorhodamine 123 flow cytometry using a leukocyte suspension spiked with a very small patch of the allergen crude extract, Dermatophagoides farinae (Der f). The mean fluorescence intensity ratio of stimulated to unstimulated samples was calculated as the neutrophil oxidative index (NOI).

RESULTS:

The Der f-specific NOI (Der f-sNOI) showed a time-dependent increase after Der f extract addition. At 15 min activation, higher Der f-sIgG levels were associated with lower Der f-sNOI values in 31 subjects (P < 0.05). This inverse relationship occurs due to the initial blocking effect of free Der f-sIgG. Additionally, neutrophil OB was nearly absent (Der f-sNOI of −1) in two cases a subject with undetectable Der f-sIgG levels and washed leukocyte suspensions deprived of Der f-sIgG.

CONCLUSION:

Allergens can trigger neutrophil OB via preexisting allergen-sIgG. Neutrophil OB can be easily measured in a leukocyte suspension spiked with the allergen. This assay can be used to diagnose IgG-mediated anaphylaxis.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Suspensions / In Vitro Techniques / Immunoglobulin G / Allergens / Respiratory Burst / Dermatophagoides farinae / Flow Cytometry / Fluorescence / Anaphylaxis / Leukocytes Type of study: Diagnostic study Language: English Journal: Blood Research Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Suspensions / In Vitro Techniques / Immunoglobulin G / Allergens / Respiratory Burst / Dermatophagoides farinae / Flow Cytometry / Fluorescence / Anaphylaxis / Leukocytes Type of study: Diagnostic study Language: English Journal: Blood Research Year: 2018 Type: Article