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Bacopa monnieri extract improves novel object recognition, cell proliferation, neuroblast differentiation, brain-derived neurotrophic factor, and phosphorylation of cAMP response element-binding protein in the dentate gyrus / 한국실험동물학회지
Laboratory Animal Research ; : 239-247, 2018.
Article in English | WPRIM | ID: wpr-718843
ABSTRACT
Bacopa monnieri is a medicinal plant with a long history of use in Ayurveda, especially in the treatment of poor memory and cognitive deficits. In the present study, we hypothesized that Bacopa monnieri extract (BME) can improve memory via increased cell proliferation and neuroblast differentiation in the dentate gyrus. BME was administered to 7-week-old mice once a day for 4 weeks and a novel object recognition memory test was performed. Thereafter, the mice were euthanized followed by immunohistochemistry analysis for Ki67, doublecortin (DCX), and phosphorylated cAMP response element-binding protein (CREB), and western blot analysis of brain-derived neurotrophic factor (BDNF). BME-treated mice showed moderate increases in the exploration of new objects when compared with that of familiar objects, leading to a significant higher discrimination index compared with vehicle-treated mice. Ki67 and DCX immunohistochemistry showed a facilitation of cell proliferation and neuroblast differentiation following the administration of BME in the dentate gyrus. In addition, administration of BME significantly elevated the BDNF protein expression in the hippocampal dentate gyrus, and increased CREB phosphorylation in the dentate gyrus. These data suggest that BME improves novel object recognition by increasing the cell proliferation and neuroblast differentiation in the dentate gyrus, and this may be closely related to elevated levels of BDNF and CREB phosphorylation in the dentate gyrus.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Plants, Medicinal / Immunohistochemistry / Blotting, Western / Cyclic AMP Response Element-Binding Protein / Cognition Disorders / Dentate Gyrus / Brain-Derived Neurotrophic Factor / Bacopa / Cell Proliferation Limits: Animals Language: English Journal: Laboratory Animal Research Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Plants, Medicinal / Immunohistochemistry / Blotting, Western / Cyclic AMP Response Element-Binding Protein / Cognition Disorders / Dentate Gyrus / Brain-Derived Neurotrophic Factor / Bacopa / Cell Proliferation Limits: Animals Language: English Journal: Laboratory Animal Research Year: 2018 Type: Article