Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus
Clinical and Experimental Vaccine Research
;
: 54-63, 2019.
Article
in English
| WPRIM
| ID: wpr-719487
ABSTRACT
PURPOSE:
The influenza B virus diverges into two antigenically distinct lineages B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity. MATERIALS ANDMETHODS:
The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured.RESULTS:
Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference.CONCLUSION:
The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Influenza B virus
/
Seasons
/
Victoria
/
Influenza Vaccines
/
Vaccines
/
Lymphocytes
/
T-Lymphocytes
/
T-Lymphocytes, Cytotoxic
/
Adenoviridae
/
Vaccination
Limits:
Animals
/
Humans
Country/Region as subject:
Oceania
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2019
Type:
Article
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