Vaccine containing G protein fragment and recombinant baculovirus expressing M2 protein induces protective immunity to respiratory syncytial virus
Clinical and Experimental Vaccine Research
;
: 43-53, 2019.
Article
in English
| WPRIM
| ID: wpr-719488
ABSTRACT
PURPOSE:
Respiratory syncytial virus (RSV) can cause serious respiratory illnesses such as pneumonia, asthma, and bronchiolitis in infants and elderly or immunocompromised individuals. An RSV vaccine has yet to be developed; only prophylactic anti-RSV antibody is commercially available. So, we investigated whether our vaccine candidate is able to induce type 1 CD4+ T helper (Th1), CD8+ T-cell responses, and protective immunity without vaccine-enhanced disease (VED) against RSV. MATERIALS ANDMETHODS:
We used RSV G protein fragment (Gcf A) with recombinant baculovirus capable of expressing the RSV M2 protein (Bac M2) as a vaccine candidate, and injected this vaccine (Gcf A/Bac M2) intramuscularly, and challenged with RSV intranasally into mice. Enzyme-linked immunosorbent assay, flow cytometry, plaque assay, and weight measurement were performed to confirm humoral immunity, cellular immunity, and protective immunity.RESULTS:
The Gcf A/Bac M2 formulation induced a stronger IgG response to Gcf A than Gcf A inoculation alone, and the ratio of IgG1/IgG2a indicated that the responses shifted predominantly to Th1. In addition, both RSV G-specific Th1 responses and RSV M2-specific CD8+ T-cell responses were induced, and G protein-associated eosinophilic infiltration was suppressed compared to the control group. Moreover, the Gcf A/Bac M2 group showed effective protection after an RSV challenge.CONCLUSION:
Bac M2 could serve as a vaccine with intrinsic adjuvant activity, and the Gcf A/Bac M2 shows promise as a vaccine candidate for inducing protective immunity without inciting VED.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Respiratory Syncytial Viruses
/
Asthma
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Bronchiolitis
/
T-Lymphocytes
/
Baculoviridae
/
GTP-Binding Proteins
/
Eosinophils
Limits:
Animals
/
Humans
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2019
Type:
Article
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