Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
Allergy, Asthma & Immunology Research
;
: 104-115, 2019.
Article
in English
| WPRIM
| ID: wpr-719508
ABSTRACT
BACKGROUND:
Elderly asthma (EA) is increasing, but the pathogenesis is unclear. This study aimed to identify EA-related biological pathways by analyzing genome-wide gene expression profiles in sputum cells.METHODS:
A total of 3,156 gene probes with significantly differential expressions between EA and healthy elderly controls were used for a hierarchical clustering of genes to identify gene clusters. Gene set enrichment analysis provided biological information, with replication from Gene Expression Omnibus expression profiles.RESULTS:
Fifty-five EA patients and 10 elderly control subjects were enrolled. Two distinct gene clusters were found. Cluster 1 (n = 35) showed a lower eosinophil proportion in sputum and less severe airway obstruction compared to cluster 2 (n = 20). The replication data set also identified 2 gene clusters (clusters 1' and 2'). Among 5 gene sets significantly enriched in cluster 1 and 3 gene sets significantly enriched in cluster 2, we confirmed that 2 were significantly enriched in the replication data set (OXIDATIVE_PHOSPHORYLATION gene set in cluster 1 and EPITHELIAL MESENCHYMAL TRANSITION gene set in cluster 2').CONCLUSIONS:
The findings of 2 distinct gene clusters in EA and different biological pathways in each gene cluster suggest 2 different pathogenesis mechanisms underlying EA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Sputum
/
Cluster Analysis
/
Gene Expression
/
Multigene Family
/
Airway Obstruction
/
Eosinophils
/
Epithelial-Mesenchymal Transition
/
Transcriptome
/
Dataset
Type of study:
Prognostic study
Limits:
Aged
/
Humans
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2019
Type:
Article
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