Induction of hypoxia-inducible factor-1alpha inhibits drug-induced apoptosis in the human leukemic cell line HL-60 / 대한혈액학회지
Korean Journal of Hematology
;
: 158-163, 2010.
Article
in English
| WPRIM
| ID: wpr-720398
ABSTRACT
BACKGROUND:
Leukemic cells originate from hypoxic bone marrow, which protects them from anti-cancer drugs. Although many factors that cause drug resistance in leukemic cells have been studied, the effect of hypoxia on drug-induced apoptosis is still poorly understood.METHODS:
In this study, we examined the effect of hypoxia on anti-leukemic drug resistance in leukemic cell lines treated with cobalt chloride (CoCl2), a hypoxia-mimetic agent. Cellular proliferation was evaluated using the methyl thiazolyl tetrazolium (MTT) assay. Flow cytometry analysis and western blots were performed to investigate apoptosis-related proteins.RESULTS:
Unlike its previously known apoptotic effect, the expression of HIF-1alpha increased the survival rate of human promyelocytic leukemia HL-60 cells when these cells were exposed to anti-leukemic drugs; these effects were mediated by heat-shock protein HSP70 and the pro-apoptotic protein Bax.CONCLUSION:
These findings may provide new insights for understanding the mechanisms underlying hypoxia and for designing new therapeutic strategies for acute myeloid leukemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Arsenicals
/
Bone Marrow
/
Drug Resistance
/
Leukemia, Myeloid, Acute
/
Leukemia
/
Proteins
/
Cell Line
/
Blotting, Western
/
Survival Rate
Limits:
Humans
Language:
English
Journal:
Korean Journal of Hematology
Year:
2010
Type:
Article
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