The Characteristics of Residual Leukocyte in Sepacell PLS-5A Filtered Platelet Concentrates / 대한혈액학회지
Korean Journal of Hematology
;
: 406-410, 1998.
Article
in Korean
| WPRIM
| ID: wpr-720414
ABSTRACT
BACKGROUND:
We evaluated residual leukocytes characteristics of white cell(WBC) reduction filter in platelet concentrates. Differential count and lymphocyte subset changes were measured before and after leukocyte filtration in platelet concentrates. MATERIAL ANDMETHODS:
Ten units of platelet concentrates were prepared and were filtered with WBC-reduction filter(Sepacell PLS 5A, Japan). After filtration of blood products, WBC and differential leukocyte count and lymphocyte subsets were counted by microscopic examination of Wright-Giemsa stained smear and Facscan(Becton-Dickinson, USA). Monoclonal antibodies used for lymphocyte subset test were CD3(FITC), CD4(FITC), CD8(PE), CDl4(PE), CDl6(PE), CDl9(PE), CD33(PE), CD56(PE), IgGl(FITC), IgG2(PE).RESULTS:
The main population of residual leukocytes after filtration was mainly lymphocytes(96.7%), and CD3 positive T lymphocytes showed 23.8% positivity of residual leukocyctes and the next were NK cell(8.7%). B lymphocytes were rarely found(<0.01%) and CD4/ CD8 ratio was within normal limits.CONCLUSION:
The leukocyte reduction filters(Sepacell PLS-5A) would be effective for prevention of platelet alloimmunization but not sure about the effect for prevention of TA GVHD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Platelets
/
B-Lymphocytes
/
T-Lymphocytes
/
Lymphocyte Subsets
/
Filtration
/
Leukocyte Count
/
Leukocytes
/
Antibodies, Monoclonal
Language:
Korean
Journal:
Korean Journal of Hematology
Year:
1998
Type:
Article
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