Measurement of HIV-1-specific T Cell Immunity by Using Recombinant Gag Protein
Infection and Chemotherapy
;
: 374-382, 2006.
Article
in Korean
| WPRIM
| ID: wpr-721398
ABSTRACT
BACKGROUND:
HIV-specific immunity, such as strong CD4+ helper and CD8+ cytotoxic T lymphocytes (CTL) responses, develops soon after HIV infection, but usually it can not control HIV replication which ultimately results in severe immune deficiency. HIV-specific CTLs, which are induced by HIV-specific CD4+ helper responses, are the key to cellular immune control of HIV. Measurement of HIV-1-specific CTLs using recombinant Gag protein may be very useful, because it is not restricted by HLA haplotype of the infected individual. MATERIALS ANDMETHODS:
Enzyme-linked immunospot (ELISPOT) assays by using recombinant Gag protein were performed to evaluate HIV-1-specific gamma-interferon cellular responses of 25 HIV-1 infected Korean patients, who had been treated at least for the prior 12 months with highly active antiretroviral therapy at Catholic University Kangnam St. Mary's Hospital.RESULTS:
The study group consisted of 25 chronically HIV-infected individuals with a median age of 51 years. The median CD4 counts were 556/mm3 (range369-994/mm3) and HIV RNA titers were < 25 copies/mL (range <25-180copies/mL). HIV-1-specific ELISPOT assay results range from 0 to 49 SFCs/2 x 10(5) PBMCs (median, 23.5 SFCs/2 x 10(5) PBMCs). CMV pp65-specific ELISPOT assay results range from 5 to 591 SFCs/2 x 10(5) PBMCs (median, 34 SFCs/2 x 10(5) PBMCs). There was no correlation between CD4 counts and HIV-1-specific SFCs measured by ELISPOT using recombinant protein.CONCLUSION:
ELISPOT assays by using recombinant Gag protein may be considerable value in the assessment of cell-mediated immunity of HIV-1 infected patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Haplotypes
/
RNA
/
T-Lymphocytes, Cytotoxic
/
Gene Products, gag
/
HIV Infections
/
HIV-1
/
HIV
/
Interferon-gamma
/
CD4 Lymphocyte Count
/
Antiretroviral Therapy, Highly Active
Limits:
Humans
Language:
Korean
Journal:
Infection and Chemotherapy
Year:
2006
Type:
Article
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