Rapid Detection of Extended Spectrum beta-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method
Infection and Chemotherapy
;
: 181-184, 2009.
Article
in English
| WPRIM
| ID: wpr-722121
ABSTRACT
A multiplex PCR method has been developed to classify extended spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type beta-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of beta-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Proteins
/
Beta-Lactamases
/
Oxytocin
/
Polymerase Chain Reaction
/
Enterobacteriaceae
/
Multiplex Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Infection and Chemotherapy
Year:
2009
Type:
Article
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