The Preparation of Frozen Red Blood Cells and Deglycerolization of Frozen RBCs with Haemonetics V50plus / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 149-155, 2002.
Article
in Korean
| WPRIM
| ID: wpr-7233
ABSTRACT
BACKGROUND:
The method utilizing high concentration glycerol is a common way to make frozen red bool cells (RBCs) for extended period, but it requires special deglycerolization equipment for washing after thawing. For economics reason, we could not have a cell washer for wahing the frozen RBCs, so attempted to use Haemonetics V50plus that we have had.METHODS:
Twelve fresh packed RBCs were cryopreserved with 40% glycerol method. After 3 months, the RBCs were thawed and washed with Haemonetics V50plus. For the evaluation of the procedure, RBC reovery rate, osmolarity, 30 min saline stability (SS), % hemolysis, ATP, 2,3-DPG, LDH, potassium, and plasma hemoglobin were tested at 24hrs after washing.RESULTS:
The RBC recovery rate was 82.1 +/- 4.5% (75.8-89.2) and two units of Frozen RBCs were under 80%. The Hb ATP and Hb 2,3-DPG of RBCs were 5.2 +/- 0.6 micro mol/g Hb (3.9-6.0) and 13.0 +/- 2.1 micro mol/g Hb (8.8-15.1). The supernatant osmalrity, potassium, plasma Hb and LDH were 352 +/- 7mosmol/kg H2O (342~367), 0.8 +/- 0.2 mmol/L (0.5~1.2), 0.8 +/- 0.3 mg/dL (0.4~1.1), 352 +/- 7 U/L (342~367). The 30min SS was 98.9 +/- 0.8.CONCLUSION:
We could successfully freeze, thaw, and wash the frozen RBCs with Haemonetics V50 plus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osmolar Concentration
/
Plasma
/
Potassium
/
Cryopreservation
/
Adenosine Triphosphate
/
2,3-Diphosphoglycerate
/
Erythrocytes
/
Glycerol
/
Hemolysis
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS