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MicroRNA-21 controls the development of osteoarthritis by targeting GDF-5 in chondrocytes
Experimental & Molecular Medicine ; : e79-2014.
Article in English | WPRIM | ID: wpr-72398
ABSTRACT
Osteoarthritis is a common cause of functional deterioration in older adults and is an immense burden on the aging population. Altered chondrogenesis is the most important pathophysiological process involved in the development of osteoarthritis. However, the molecular mechanism underlying the regulation of chondrogenesis in patients with osteoarthritis requires further elucidation, particularly with respect to the role of microRNAs. MiR-21 expression in cartilage specimens was examined in 10 patients with knee osteoarthritis and 10 traumatic amputees. The effect of miR-21 on chondrogenesis was also investigated in a chondrocyte cell line. The effect of miR-21 on the expression of growth differentiation factor 5 (GDF-5) was further assessed by luciferase reporter assay and western blot. We found that endogenous miR-21 is upregulated in osteoarthritis patients, and overexpression of miR-21 could attenuate the process of chondrogenesis. Furthermore, we identified GDF-5 as the direct target of miR-21 during the regulation of chondrogenesis. Our data suggest that miR-21 has an important role in the pathogenesis of osteoarthritis and is a potential therapeutic target.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoarthritis / Cartilage / Case-Control Studies / Up-Regulation / Cell Line / Chondrocytes / MicroRNAs / Growth Differentiation Factor 5 Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: Experimental & Molecular Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoarthritis / Cartilage / Case-Control Studies / Up-Regulation / Cell Line / Chondrocytes / MicroRNAs / Growth Differentiation Factor 5 Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: Experimental & Molecular Medicine Year: 2014 Type: Article