Apoptosis and remodeling in adriamycin-induced cardiomyopathy rat model / 소아과
Korean Journal of Pediatrics
;
: 365-372, 2017.
Article
in English
| WPRIM
| ID: wpr-72672
ABSTRACT
PURPOSE:
The mechanism for the pathogenesis of adriamycin (ADR)-induced cardiomyopathy is not yet known. Different hypotheses include the production of free radicals, an interaction between ADR and nuclear components, and a disruption in cardiac-specific gene expression. Apoptosis has also been proposed as being involved in cardiac dysfunction. The purpose of this study was to determine if apoptosis might play a role in ADR-induced cardiomyopathy.METHODS:
Male Sprague-Dawley rats were separated into 2 groups the control group (C group) and the experimental group (ADR 5 mg/wk for 3 weeks through intraperitoneal injections; A group). Echocardiographic images were obtained at week 3. Changes in caspase-3, B-cell leukemia/lymphoma (Bcl)-2, Bcl-2-associated X (Bax), interleukin (IL)-6, tumor necrosis factor-α, brain natriuretic peptide (BNP), troponin I, collagen 1, and collagen 3 protein expression from the left ventricle tissues of C and A group rats were determined by Western blot.RESULTS:
Ascites and heart failure as well as left ventricular hypertrophy were noted in the A group. Ejection fraction and shortening fraction were significantly lower in the A group by echocardiography. The expression of caspase-3, Bax, IL-6, BNP, collagen 1, and collagen 3 were significantly higher in the A group as compared with the C group. Protein expression of Bcl-2 decreased significantly in the A group compared with the C group.CONCLUSION:
ADR induced an upregulation of caspase-3, Bax, IL-6, and collagen, as well as a depression in Bcl-2. Thus, apoptosis and fibrosis may play an important role in ADR-induced cardiomyopathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ascites
/
Fibrosis
/
B-Lymphocytes
/
Echocardiography
/
Doxorubicin
/
Gene Expression
/
Up-Regulation
/
Blotting, Western
/
Collagen
/
Interleukins
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2017
Type:
Article
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