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The Characteristics of Supramammillary Cells Projecting to the Hippocampus in Stress Response in the Rat
The Korean Journal of Physiology and Pharmacology ; : 17-24, 2012.
Article in English | WPRIM | ID: wpr-727563
ABSTRACT
The hypothalamus-pituitary-adrenocortex (HPA) axis is the central mediator of the stress response. The supramammillary (SuM) region is relatively unique among the hypothalamic structures in that it sends a large, direct projection to the hippocampal formation. It has been shown that mild stress could activate the SuM cells that project to the hippocampus. However, the role of these cell populations in modulating the stress response is not known. The present study examined the effect of stress on different populations of SuM cells that project to the hippocampus by injecting the fluorescent retrograde tracer, fluorogold (FG), into the hippocampus and utilizing the immunohistochemistry of choline acetyltransferase (ChAT), corticotrophin releasing factor (CRF), serotonin (5-HT), glutamate decarboxylase (GAD), tyrosine hydroxylase (TH) and NADPH-d reactivity. Immobilization (IMO) stress (2 hr) produced an increase in the expression of ChAT-immunoreactivity, and tended to increase in CRF, 5-HT, GAD, TH-immunoreactivity and nitric oxide (NO)-reactivity in the SuM cells. Fifty-three percent of 5-HT, 31% of ChAT and 56% of CRF cells were double stained with retrograde cells from the hippocampus. By contrast, a few retrogradely labeled cells projecting to the hippocampus were immunoreactive for dopamine, gamma-aminobutyric acid (GABA) and NO. These results suggest that the SuM region contains distinct cell populations that differentially respond to stress. In addition, the findings suggest that serotonergic, cholinergic and corticotropin releasing cells projecting to the hippocampus within the SuM nucleus may play an important role in modulating stress-related behaviors.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Axis, Cervical Vertebra / Tyrosine 3-Monooxygenase / Immunohistochemistry / Dopamine / Serotonin / Choline O-Acetyltransferase / Adrenocorticotropic Hormone / Gamma-Aminobutyric Acid / Glutamate Decarboxylase / Hippocampus Limits: Animals Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Axis, Cervical Vertebra / Tyrosine 3-Monooxygenase / Immunohistochemistry / Dopamine / Serotonin / Choline O-Acetyltransferase / Adrenocorticotropic Hormone / Gamma-Aminobutyric Acid / Glutamate Decarboxylase / Hippocampus Limits: Animals Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2012 Type: Article