Binding Specificity of Philyra pisum Lectin to Pathogen-Associated Molecular Patterns, and Its Secondary Structure
The Korean Journal of Physiology and Pharmacology
;
: 547-551, 2013.
Article
in English
| WPRIM
| ID: wpr-727603
ABSTRACT
We recently reported a Philyra pisum lectin (PPL) that exerts mitogenic effects on human lymphocytes, and its molecular characterization. The present study provides a more detailed characterization of PPL based on the results from a monosaccharide analysis indicating that PPL is a glycoprotein, and circular dichroism spectra revealing its estimated alpha-helix, beta-sheet, beta-turn, and random coil contents to be 14.0%, 39.6%, 15.8%, and 30.6%, respectively. These contents are quite similar to those of deglycosylated PPL, indicating that glycans do not affect its intact structure. The binding properties to different pathogen-associated molecular patterns were investigated with hemagglutination inhibition assays using lipoteichoic acid from Gram-positive bacteria, lipopolysaccharide from Gram-negative bacteria, and both mannan and beta-1,3-glucan from fungi. PPL binds to lipoteichoic acids and mannan, but not to lipopolysaccharides or beta-1,3-glucan. PPL exerted no significant antiproliferative effects against human breast or bladder cancer cells. These results indicate that PPL is a glycoprotein with a lipoteichoic acid or mannan-binding specificity and which contains low and high proportions of alpha-helix and beta-structures, respectively. These properties are inherent to the innate immune system of P. pisum and indicate that PPL could be involved in signal transmission into Gram-positive bacteria or fungi.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polysaccharides
/
Teichoic Acids
/
Urinary Bladder Neoplasms
/
Breast
/
Glycoproteins
/
Lymphocytes
/
Lipopolysaccharides
/
Sensitivity and Specificity
/
Circular Dichroism
/
Beta-Glucans
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2013
Type:
Article
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