Decreasing Effect of Lidocaine.HCl on the Thickness of the Neuronal and Model Membrane
The Korean Journal of Physiology and Pharmacology
;
: 253-257, 2013.
Article
in English
| WPRIM
| ID: wpr-727722
ABSTRACT
This study examined the mechanism of action of a local anesthetic, lidocaine.HCl. Energy transfer between the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, and the hydrophobic fluorescent probe, 1,3-di(1-pyrenyl) propane, was used to determine the effect of lidocaine.HCl on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex, and liposomes of the total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. The thickness (D) of the intact SPMV, SPMVTL and SPMVPL were 1.044+/-0.008, 0.914+/-0.005 and 0.890+/-0.003 (arbitrary units, n=5) at 37degrees C (pH 7.4), respectively. Lidocaine.HCl decreased the thickness of the neuronal and model membrane lipid bilayers in a dose-dependent manner with a significant decrease in the thickness, even at 0.1 mM. The decreasing effect of lidocaine.HCl on the membrane thickness might be responsible for some, but not all of its anesthetic action.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipids
/
Propane
/
Cell Membrane
/
Cerebral Cortex
/
Energy Transfer
/
Lipid Bilayers
/
Liposomes
/
Anilino Naphthalenesulfonates
/
Membranes
/
Neurons
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2013
Type:
Article
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