The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes
The Korean Journal of Physiology and Pharmacology
;
: 121-127, 2000.
Article
in English
| WPRIM
| ID: wpr-727747
ABSTRACT
Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0~400 micrometer) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400 micrometer GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose-dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Bile
/
Bile Acids and Salts
/
Lipid Peroxidation
/
Cholestasis
/
Cell Death
/
Apoptosis
/
Hepatocytes
/
Electrophoresis, Agar Gel
/
Glutathione
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2000
Type:
Article
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