Role of phospholipase A2 in oxidant-induced alteration in phosphate transport in primary cultured rabbit renal proximal tubule cells
The Korean Journal of Physiology and Pharmacology
;
: 601-609, 1998.
Article
in English
| WPRIM
| ID: wpr-727756
ABSTRACT
The present study was undertaken to examine the role of phospholipase A2 (PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose-dependent fashion. A PLA2 inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na+-K+-ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA2 activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipases
/
Quinacrine
/
Lipid Peroxidation
/
Cell Survival
/
Arachidonic Acid
/
Staurosporine
/
Tert-Butylhydroperoxide
/
Phospholipases A2
/
Glucose
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1998
Type:
Article
Similar
MEDLINE
...
LILACS
LIS