Effects of PCB Congeners in Rodent Neuronal Cells in Culture
The Korean Journal of Physiology and Pharmacology
;
: 9-15, 2005.
Article
in English
| WPRIM
| ID: wpr-727775
ABSTRACT
We attempted to analyze the mechanism of polychlorinated biphenyl (PCB) -induced neurotoxicity and identify the target molecules in the neuronal cells for PCBs. Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old Sprague Dawley (SD) rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. Only non-coplanar PCBs at a high dose showed a significant increase of total protein kinase C (PKC) activity at phobol 12, 13-dibutyrate ([3H]PDBu) binding assay, indicating that non-coplanar PCBs are more neuroactive than coplanar PCBs in neuronal cells. PKC isozymes were immunoblotted with the selected monoclonal antibodies. PKC-alpha, delta, and epsilon were activated with non-coplanar PCB exposure. Receptor for activated C kinase-1 (RACK-1), anchoring protein for activated PKC, was more induced with exposure to coplanar PCBs than non-coplanar PCBs. Reverse transcription PCR (RT-PCR) analysis showed induction of neurogranin (RC-3) and growth associated protein-43 (GAP-43) mRNA with non-coplanar PCBs. The results indicate that these factors may be useful biomarkers for differentiating non-coplanar PCBs from coplanar PCBs. The present study demonstrated that non-coplanar PCBs are more neuroactive congeners than coplanar PCBs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rodentia
/
Protein Kinase C
/
RNA, Messenger
/
Biomarkers
/
Cerebellum
/
Polymerase Chain Reaction
/
Polychlorinated Biphenyls
/
Reverse Transcription
/
Neurogranin
/
Isoenzymes
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2005
Type:
Article
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