Effects of Proinflammatory Cytokines and Natural Products on Mucin Release from Cultured Hamster Tracheal Surface Epithelial Cells
The Korean Journal of Physiology and Pharmacology
;
: 329-333, 2004.
Article
in English
| WPRIM
| ID: wpr-727781
ABSTRACT
In this study, we investigated whether TNF-alpha, IL-1beta, CTMA (carboxymethyl trimethylammonium) and LPD (Lup-20[29]-ene-3beta, 28-diol) affect mucin release from airway goblet cells and compared the activities of these agents with the inhibitory action of PLL and the stimulatory action of ATP on mucin release. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on 3H-mucin release. The results were as follows TNF-alpha, CTMA and LPD increased mucin release at the highest concentration, but IL-1beta did not. We conclude that CTMA and LPD can stimulate mucin release by directly acting on airway mucin-secreting cells, and suggest that these agents should be further investigated for the possible use as mild expectorants during the treatment of chronic airway diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biological Products
/
Adenosine Triphosphate
/
Cytokines
/
Tumor Necrosis Factor-alpha
/
Goblet Cells
/
Epithelial Cells
/
Expectorants
/
Mucins
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2004
Type:
Article
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