Involvement of peripheral benzodiazepine receptor on contractility of canine trachealis muscle

ABSTRACT

Non-neuronal high affinity binding sites for benzodiazepines have been found in many peripheral tissues including cardiac muscle and vascular smooth muscle, and have been designated as 'peripheral benzodiazepine receptor'. Benzodiazepines have been shown to induce relaxation of the ileal, vesical, and uterine smooth muscles. However, it is still unclear about possible involvement of peripheral benzodiazepine receptor on the contractility of trachealis muscle. This study was performed to investigate the role of the peripheral benzodiazepine receptor on the contractility of canine trachealis muscle. Canine trachealis muscle strips of 15 mm long were suspended in an isolated organ bath containing 1 ml of physiological salt solution maintained at 37degreeC, and aerated with 95% O2/5% CO2. Isometric myography was performed, and the results of the experiments were as follows Ro5-4684, FGIN-1-27 and clonazepam reduced a basal tone of isolated canine trachealis muscle strip concentration dependently, relaxant actions of Ro5-4684 and FGIN-1-27 were antagonized by PK11195, a peripheral benzodiazepine receptor antagonist. Flumazenil, a central type antagonist, did not antagonize the relaxant action of peripheral type agonists. Saturation binding assay of (3H)Ro5-4864 showed a high affinity (Kd = 5.33 +/- 1.27nM, Bmax = 867.3 +/- 147.2 fmol/mg protein) binding site on the canine trachealis muscle. Ro5-4684 suppressed the bethanechol-, 5-hydroxytryptamine- and histamine-induced contractions. Platelet activating factor (PAF) exerted strong and prolonged contraction in trachealis muscle strip. Strong tonic contraction by PAF was attenuated by Ro 5-4684, but not by WEB 2086, a PAF antagonist. Based on these results, it is concluded that the peripheral benzodiazepine receptor mediates the inhibitory regulation of contractility of canine trachealis muscle.