Immunostimulatory Effects of beta-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes
The Korean Journal of Physiology and Pharmacology
;
: 225-230, 2012.
Article
in English
| WPRIM
| ID: wpr-728096
ABSTRACT
We investigated the effects of beta-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that beta-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. beta-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that beta-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of beta-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, beta-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and beta-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that beta-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of beta-glucan in research fields.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trypan Blue
/
Enzyme-Linked Immunosorbent Assay
/
Lymphocytes
/
Interleukin-7
/
Interleukin-2
/
Cell Death
/
Dependency, Psychological
/
Diminazene
/
Plasmodiophorida
/
Paenibacillus
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2012
Type:
Article
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