Alteration of 4-aminopyridine-sensitive, voltage-dependent K+-channel in arterial smooth muscle cells of one-kidney, one-clip Goldblatt hypertensive rats
The Korean Journal of Physiology and Pharmacology
;
: 385-391, 2000.
Article
in English
| WPRIM
| ID: wpr-728138
ABSTRACT
Using the patch-clamp technique, we investigated the alteration of 4-aminopyridine(4-AP)-sensitive, voltage-dependent K+ channel (KV) in the mesenteric arterial smooth muscle cell (MASMC) of renovascular hypertensive model, one-kidney one-clip Goldblatt hypertensive rat (GBH). To isolate KV current, internal pipette solution contained 5 mM ATP and 10 mM EGTA. Under these condition, MASMC was depolarized by 4-AP, but charybdotoxin did not affect membrane potential. Membrane potential of hypertensive cell (- 40.3 +/- 3.2 mV) was reduced when compared to that of normotensive cell (-59.5 +/- 2.8 mV). Outward K+ current of hypertensive cell was significantly reduced when compared to normotensive cell. At 60 mV, the outward currents were 19.10 +/- 1.91 and 14.06 +/- 1.05 pA/pF in normotensive cell and hypertensive cell respectively. 4-AP-sensitive K+ current was also smaller in hypertensive cell (4.28 +/- 0.38 pA/pF) than in normotensive cell (7.65 +/- 0.52 pA/pF). The values of half activation voltage (V1/2) and slope factor (k1) as well as the values of half inactivation voltage (V1/2) and slope factor (k1) were virtually similar between GBH and NTR. These results suggest that the decrease of 4-AP-sensitive K+ current contributes to a depolarization of membrane potential, which leads to development of vascular tone in GBH.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Adenosine Triphosphate
/
Egtazic Acid
/
Patch-Clamp Techniques
/
Charybdotoxin
/
Myocytes, Smooth Muscle
/
Membrane Potentials
/
Muscle, Smooth
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2000
Type:
Article
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