Extracellular ATP stimulates Na+ and Cl- transport through the activation of multiple purinergic receptors on the apical and basolateral membranes in M-1 mouse cortical collecting duct cells
The Korean Journal of Physiology and Pharmacology
;
: 231-241, 2001.
Article
in English
| WPRIM
| ID: wpr-728213
ABSTRACT
The mammalian cortical collecting duct (CCD) plays a major role in regulating renal NaCl reabsorption, which is important in Na+ and Cl- homeostasis. The M-1 cell line, derived from the mouse cortical collecting duct, has been used as a mammalian model of the study on the electrolytes transport in CCD. M-1 cells were grown on collagen-coated permeable support and short circuit current (Isc) was measured. M-1 cells developed amiloride-sensitive current 5apprx7 days after seeding. Apical and basolateral addition of ATP induced increase in Isc in M-1 cells, which was partly retained in Na+/-free or Cl--free solution, indicating that ATP increased Na+ absorption and Cl- secretion in M-1 cells. Cl- secretion was mediated by the activation of apical cystic fibrosis transmembrane regulator (CFTR) chloride channels and Ca2+/-activated chloride channels, but Na+ absorption was not mediated by activation of epithelal sodium channel (ENaC). ATP increased cAMP content in M-1 cells. The RT-PCR analysis demonstrated that M-1 cells express P2Y2, P2X3 and P2Y4 receptors. These results showed that ATP regulates Na+ and Cl- transports via multiple P2 purinoceptors on the apical and basolateral membranes in M-1 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sodium Channels
/
Cell Line
/
Adenosine Triphosphate
/
Receptors, Purinergic
/
Receptors, Purinergic P2
/
Chloride Channels
/
Cystic Fibrosis
/
Absorption
/
Electrolytes
/
Homeostasis
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2001
Type:
Article
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