The Protective Effect of Quercetin-3-O-beta-D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells
The Korean Journal of Physiology and Pharmacology
;
: 319-326, 2011.
Article
in English
| WPRIM
| ID: wpr-728324
ABSTRACT
Quercetin-3-O-beta-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. We aimed to explore its protective effect against ethanol-induced cell damage and the mechanism involved in the effect in feline esophageal epithelial cells (EEC). Cell viability was tested and 2',7'-dichlorofluorescin diacetate assay was used to detect intracellular H2O2 production. Western blotting analysis was performed to investigate MAPK activation and interleukin 6 (IL-6) expression. Exposure of cells to 10% ethanol time-dependently decreased cell viability. Notably, exposure to ethanol for 30 min decreased cell viability to 43.4%. When cells were incubated with 50 microM QGC for 12 h prior to and during ethanol treatment, cell viability was increased to 65%. QGC also inhibited the H2O2 production and activation of ERK 1/2 induced by ethanol. Pretreatment of cells with the NADPH oxidase inhibitor, diphenylene iodonium, also inhibited the ethanol-induced ERK 1/2 activation. Treatment of cells with ethanol for 30 or 60 min in the absence or presence of QGC exhibited no changes in the IL-6 expression or release compared to control. Taken together, the data indicate that the cytoprotective effect of QGC against ethanol-induced cell damage may involve inhibition of ROS generation and downstream activation of the ERK 1/2 in feline EEC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Onium Compounds
/
Quercetin
/
Cell Survival
/
Blotting, Western
/
Interleukin-6
/
NADPH Oxidases
/
Rumex
/
Ethanol
/
Epithelial Cells
/
European Union
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2011
Type:
Article
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