Effects of ethanol on Na+-dependent solute uptake in rabbit renal brush-border membrane vesicles
The Korean Journal of Physiology and Pharmacology
;
: 191-198, 1999.
Article
in English
| WPRIM
| ID: wpr-728417
ABSTRACT
This study was undertaken to examine the effect of ethanol on Na+-dependent transport systems (glucose, phosphate, and dicarboxylate) in renal brush-border membrane vesicles (BBMV). Ethanol inhibited Na+-dependent uptakes of glucose, phosphate, and succinate in a dose-dependent manner, but not the uptakes of Na+-independent. The H+/TEA antiport was reduced by 8% ethanol. Kinetic analysis showed that ethanol caused a decrease in Vmax of three transport systems, leaving Km values unchanged. Ethanol decreased phlorizin binding, which was closely correlated with the decrease in Vmax of Na+-glucose uptake. These results indicate that ethanol inhibits Na+-dependent uptakes of glucose, phosphate, and dicaboxylate and that the reduction in Vmax of Na+-glucose uptake is caused by a decrease in the number of active carrier proteins in the membrane.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phlorhizin
/
Carrier Proteins
/
Ion Transport
/
Succinic Acid
/
Ethanol
/
Glucose
/
Membranes
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS