Role of phospholipase A2 in hypoxia-induced renal cell injury
The Korean Journal of Physiology and Pharmacology
;
: 93-100, 1999.
Article
in English
| WPRIM
| ID: wpr-728426
ABSTRACT
The present study was designed to assess the roles of PLA2 activation and arachidonic acid (AA) metabolites in hypoxia-induced renal cell injury. Hypoxia increased LDH release in a dose-dependent manner in rabbit renal cortical slices, and this increase was significant after 20-min hypoxia. The hypoxia-induced LDH release was prevented by amino acids, glycine and alanine, and extracellular acidosis (pH 6.0). Buffering intracellular Ca2+ by a chelator, but not omission of Ca2+ in the medium produced a significant reduction in hypoxia-induced LDH release. The effect of hypoxia was blocked by PLA2 inhibitors, mepacrine, butacaine, and dibucaine. A similar effect was observed by a 85-kD cPLA2 inhibitor AACOCF3. AA increased hypoxia-induced LDH release, and albumin, a fatty acid absorbent, prevented the LDH release, suggesting that free fatty acids are involved in hypoxia-induced cell injury. These results suggest that PLA2 activation and its metabolic products play important roles in pathogenesis of hypoxia-induced cell injury in rabbit renal cortical slices.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipases
/
Quinacrine
/
Acidosis
/
Arachidonic Acid
/
Dibucaine
/
Alanine
/
Phospholipases A2
/
Fatty Acids, Nonesterified
/
Amino Acids
/
Glycine
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1999
Type:
Article
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