Activation of SAPK and increase in Bak levels during ceramide and indomethacin-induced apoptosis in HT29 cells
The Korean Journal of Physiology and Pharmacology
;
: 75-82, 1999.
Article
in English
| WPRIM
| ID: wpr-728428
ABSTRACT
It has been reported that activation of sphingomyelin pathway and nonsteroidal anti-inflammatory drugs (NSAIDS) inhibit the promotion of colon carcinoma. Ceramide, a metabolite of sphingomyelin, and indomethacin were shown to induce apoptosis in colon carcinoma cells. However, the mechanisms of ceramide- and indomethacin-induced apoptosis in the colon carcinoma cells are not clearly elucidated. Recent studys showed that indomethacin-induced apoptosis in colon cancer cells through the cyclooxygenase-independent pathways, and that may be mediated by generation of ceramide. In this study, we compared effects of ceramide and indomethacin on important modulators of apoptotic processes in HT29 cells, a human colon cancer cell line. Ceramide and indomethacin induced apoptosis dose- and time-dependently. Ceramide and indomethacin increased stress-activated protein kinase (SAPK) activity, and decreased mitogen-activated protein kinase (MAPK) activity. The expression of Bak was increased by the treatment of ceramide and indomethacin. The expression of other Bcl-2 related proteins (Mcl-1, Bcl-XL, Bax) which were known to be expressed in colon epithelial cells was not changed during the ceramide- and indomethacin-induced apoptosis. Our results suggest that ceramide and indomethacin share common mechanisms for induction of apoptosis in HT29 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Kinases
/
Cell Line
/
Indomethacin
/
Apoptosis
/
Colon
/
Colonic Neoplasms
/
HT29 Cells
/
Epithelial Cells
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1999
Type:
Article
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