Anti-Apoptotic Effect of Rheum undulatum Water Extract in Pancreatic beta-cell Line, HIT-T15
The Korean Journal of Physiology and Pharmacology
;
: 51-55, 2004.
Article
in English
| WPRIM
| ID: wpr-728503
ABSTRACT
Sopungsungi-won has been used as a traditional medicine for diabetes and it has been proved to be a potential remedy for type 2 diabetes mellitus. We previously reported that water extract of Sopungsungi-won exhibits anti-diabetic effects both in vivo and in vitro experiments. In the present study, we have chosen to examined anti-apoptotic effect of Rheum undulatum, which is the main component of Sopungsungi-won, on pancreatic beta-cells, HIT-T15, against hydrogen peroxide (H2O2). oxidative stress. To investigate the anti-apoptotic effect of Rheum undulatum water extract (RUWE) against H2O2-induced apoptosis in pancreatic beta-cell line of hamster, HIT-T15, MTT assay, DAPI staining, TUNEL assay, RT-PCR and caspase-3 enzyme assay were performed. The morphological analysis demonstrated that cells treated with H2O2 exhibited classical apoptotic features, while such changes was reduced in cells pre-treated with RUWE. In addition, RUWE pre-treated cells prior to H2O2 treatment induced increase of levels of bcl-2 expression and decrease of caspase-3 enzyme activity compared to cells treated with H2O2 only. These results provide the possibility of usage of RU in patients with progressively deteriorated diabetes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rheum
/
Water
/
Apoptosis
/
Oxidative Stress
/
In Situ Nick-End Labeling
/
Diabetes Mellitus, Type 2
/
Caspase 3
/
Enzyme Assays
/
Hydrogen Peroxide
/
Medicine, Traditional
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2004
Type:
Article
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