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Nitric Oxide Donor, NOR-3, Increased Expression of Cyclooxygenase-2, but not of Cyclooxygenase-1 in Cultured VSMC
The Korean Journal of Physiology and Pharmacology ; : 161-165, 2006.
Article in English | WPRIM | ID: wpr-728563
ABSTRACT
NO and cyclooxygenase-2 (COX-2) are contributes to vascular inflammation induced by various stimulation. The mechanism, which explains a linkage between NO and COX-2, could be of importance in promoting pathophysiological conditions of vessel. We investigated the effects of NO donors on the COX-1 and COX-2 mRNA/protein expression, as well as the nitrite production in culture medium of vascular smooth muscle cell (VSMC). VSMC was primarily cultured from thoracic aorta of rat. In this experiments, COX-1 and COX-2 mRNA/protein expressions were analysed and nitrite productions were investigated using Griess reagent. VSMC did not express COX-2 protein in basal condition (Non-lipopolysaccharide (LPS) stimulated). In LPS-stimulated experiments, after 3 hours of NO donor pretreatment, LPS 10 microgram/ml was treated for 24 hours. COX-1 protein expressions were unchanged by SNP and NOR-3. NOR-3 significantly increased COX-2 mRNA/protein expression under LPS stimulation. In contrast, SNP did not increase COX-2 mRNA/protein expression under LPS stimulation. Nitrite production was higher in NOR-3 treatment than SNP treatment under LPS stimulation. These results suggest that the expression of COX-2 in VSMC is regulated by NOR-3, COX-2 expressions were depending on the types of NO donor and LPS stimulation in VSMC.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Aorta, Thoracic / Tissue Donors / Cyclooxygenase 1 / Cyclooxygenase 2 / Inflammation / Muscle, Smooth, Vascular / Nitric Oxide Limits: Animals / Humans Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Aorta, Thoracic / Tissue Donors / Cyclooxygenase 1 / Cyclooxygenase 2 / Inflammation / Muscle, Smooth, Vascular / Nitric Oxide Limits: Animals / Humans Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2006 Type: Article