Comparison of regulatory action of cAMP and cGMP on the activation of neutrophil responses
The Korean Journal of Physiology and Pharmacology
;
: 97-105, 1997.
Article
in English
| WPRIM
| ID: wpr-728643
ABSTRACT
The regulatory role of cyclic nucleotides in the expression of neutrophil responses has been examined. fMLP-stimulated superoxide production in neutrophils was inhibited by dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), histamine, adenosine + theophylline, cAMP elevating agents, and 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP) and sodium nitroprusside, cGMP elevating agents. Staurosporine, a protein kinase C inhibitor, genistein, a protein tyrosine kinase inhibitor and chlorpromazine, a calmodulin inhibitor, inhibited superoxide production by fMLP, but they did not further affect the action of DBcAMP on the stimulatory action of fMLP. DBcAMP, histamine, adenosine + theophylline and genistein inhibited myeloperoxidease release evoked by fMLP, whereas BrcGMP, sodium nitroprusside and staurosporine did not affect it. The elevation of (Ca2+)-i evoked by fMLP was inhibited by genistein and chlorpromazine but was not affected by staurosporine. DBcAMP exerted little effect on the initial peak in (Ca2+)-i response to fMLP but effectively inhibited the sustained rise. On the other hand, BrcGMP significantly inhibited both phases. fMLP-induced Mn-2+ influx was inhibited by either DBcAMP or BrcGMP. These results suggest that fMLP-stimulated neutrophil responses may be regulated by cAMP more than cGMP. cAMP and cGMP appear not affect stimulated responses by direct protein kinase C activation. Their regulatory action on the stimulated neutrophil responses may be not influenced by other activation processes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Theophylline
/
Protein Kinase C
/
Protein-Tyrosine Kinases
/
Calmodulin
/
Nitroprusside
/
Histamine
/
Adenosine
/
Chlorpromazine
/
Superoxides
/
Bucladesine
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
1997
Type:
Article
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