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Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients
The Korean Journal of Physiology and Pharmacology ; : 337-342, 2008.
Article in English | WPRIM | ID: wpr-728666
ABSTRACT
Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10th passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K+ currents, including noise-like Ca+2-activated K+ current (IKCa), a transient outward K+ current (Ito), a delayed rectifier K+ current (IKDR), and an inward-rectifier K+ current (Kir) were heterogeneously present in these cells, and a TTX-sensitive Na+ current (INa,TTX) was also recorded. In the RT-PCR analysis, Kv1.1, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, INa,TTX showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Stem Cells / Bone Marrow / Mesenchymal Stem Cells / Amyotrophic Lateral Sclerosis / Ion Channels Limits: Humans Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Stem Cells / Bone Marrow / Mesenchymal Stem Cells / Amyotrophic Lateral Sclerosis / Ion Channels Limits: Humans Language: English Journal: The Korean Journal of Physiology and Pharmacology Year: 2008 Type: Article