PKA-Mediated Regulation of B/K Gene Transcription in PC12 Cells
The Korean Journal of Physiology and Pharmacology
;
: 333-339, 2005.
Article
in English
| WPRIM
| ID: wpr-728711
ABSTRACT
B/K protein is a novel protein containing double C2-like domains. We examined the specific signaling pathway that regulates the transcription of B/K in PC12 cells. When the cells were treated with forskolin (50microM), B/K mRNA and protein levels were time-dependently decreased, reaching the lowest level at 3 or 4 hr, and thereafter returning to the control level. Chemicals such as dibutyryl-cAMP, cell- permeable cyclic AMP (cAMP) analogue and CGS21680, adenosine receptor A2A agonist, also repressed the B/K transcription. However, 1, 9-dideoxyforskolin did not show inhibitory effect on B/K transcription, suggesting direct involvement of cAMP in the forskolin-induced inhibition of B/K transcription. Effect of forskolin, dibutyryl cAMP and CGS21680 was significantly reduced in PKA-deficient PC12 cell line (PC12-123.7). One cAMP-response element (CRE) -like sequence (B/K CLS) was found in the promoter region of B/K DNA, and electrophoretic mobility shift assay indicated its binding to CREM and CREB. Forskolin significantly suppressed the promoter activity in CHO-K1 cells transfected with the constructs containing B/K CLS, but not with the construct in which B/K CLS was mutated (AC TG). Taken together, we suggest that the transcription of B/K gene in PC12 cells may be regulated by PKA-dependent mechanism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Colforsin
/
DNA
/
RNA, Messenger
/
Promoter Regions, Genetic
/
PC12 Cells
/
Receptors, Purinergic P1
/
Cyclic AMP-Dependent Protein Kinases
/
Cyclic AMP
/
Electrophoretic Mobility Shift Assay
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2005
Type:
Article
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