Effect of staurosporine on the long-term secretion of catecholamines induced by various secretagogues in cultured bovine adrenal medullary chromaffin cells
The Korean Journal of Physiology and Pharmacology
;
: 503-510, 2001.
Article
in English
| WPRIM
| ID: wpr-728780
ABSTRACT
Long-term treatment of cultured bovine adrenal medullary chromaffin (BAMC) cells with arachidonic acid (100 muM), angiotesnin II (100 nM), prostaglandin E2 (PGE2; 10 muM), veratridine (2 muM) or KCl (55 mM) for 24 hrs increased both norepinephrine and epinephrine levels in the supernatant. Pretreatment with staurosporine (10 nM), a protein kinase C (PKC) inhibitor, completely blocked increases of norepinephrine and epinephrine secretion induced by arachidonic acid, angiotensin II, PGE2, veratridine or KCl. In addition, K252a, another PKC inhibitor whose structure is similar to that of staurosporine, effectively attenuated both norepinephrine and epinephrine secretion induced by arachidonic acid. However, K252a did not affect the catecholamine secretion induced by angiotensin II, PGE2, veratridine or KCl. Our results suggest that staurosporine may inhibit long-term catecholamine secretion induced by various secretagogues in a mechanism other than inhibiting PKC signaling. Furthermore, long-term secretion of catecholamines induced by arachidonic acid may be dependent on PKC pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Veratridine
/
Protein Kinase C
/
Angiotensin II
/
Dinoprostone
/
Catecholamines
/
Epinephrine
/
Norepinephrine
/
Arachidonic Acid
/
Chromaffin Cells
/
Staurosporine
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2001
Type:
Article
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