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Assessment of Collagen-Induced Arthritis Using Cyanine 5.5 Conjugated with Hydrophobically Modified Glycol Chitosan Nanoparticles: Correlation with 18F-Fluorodeoxyglucose Positron Emission Tomography Data
Korean Journal of Radiology ; : 450-457, 2012.
Article in English | WPRIM | ID: wpr-72929
ABSTRACT

OBJECTIVE:

To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). MATERIALS AND

METHODS:

We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 microL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice.

RESULTS:

The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 +/- 0.76 and 0.79 +/- 0.71, respectively, for CIA mice; and 0.57 +/- 0.17 and 0.54 +/- 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 +/- 1.54 x 10(5) and 2.75 +/- 1.51 x 10(5), respectively, for CIA mice, and 1.22 +/- 0.27 x 10(5) and 0.88 +/- 0.24 x 10(5), respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice.

CONCLUSION:

NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Arthritis, Experimental / Carbocyanines / Statistics, Nonparametric / Microscopy, Confocal / Radiopharmaceuticals / Fluorodeoxyglucose F18 / Chitosan / Positron-Emission Tomography / Nanoparticles / Injections, Intravenous Type of study: Screening study Limits: Animals Language: English Journal: Korean Journal of Radiology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Arthritis, Experimental / Carbocyanines / Statistics, Nonparametric / Microscopy, Confocal / Radiopharmaceuticals / Fluorodeoxyglucose F18 / Chitosan / Positron-Emission Tomography / Nanoparticles / Injections, Intravenous Type of study: Screening study Limits: Animals Language: English Journal: Korean Journal of Radiology Year: 2012 Type: Article