Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa
Mycobiology
;
: 159-163, 2013.
Article
in English
| WPRIM
| ID: wpr-729419
ABSTRACT
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and beta-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in beta-glucan productivity by producing 0.254 and 0.236 mg soluble beta-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble beta-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sprains and Strains
/
Mass Screening
/
Mutagenesis
/
Mesylates
/
Agaricales
/
Efficiency
/
Methyl Methanesulfonate
Type of study:
Screening study
Language:
English
Journal:
Mycobiology
Year:
2013
Type:
Article
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