Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis
Mycobiology
;
: 108-112, 2010.
Article
in English
| WPRIM
| ID: wpr-729537
ABSTRACT
To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1 + null mutant and 4 proteins successfully identified glycolipid anchored surface precursor, beta-glucosidase (Psu1), cell surface protein, glucan 1,3-beta-glucosidase (Bgl2), and exo-1,3 beta-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Schizosaccharomyces
/
Acrylic Resins
/
Proteins
/
Cell Wall
/
Beta-Glucosidase
/
Proteome
/
Databases, Protein
/
Glucan 1,3-beta-Glucosidase
/
Electrophoresis, Polyacrylamide Gel
Language:
English
Journal:
Mycobiology
Year:
2010
Type:
Article
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